Role of structural specificity of ZnO particles in preserving functionality of proteins in their corona

Abstract Reconfiguration of protein conformation in a micro and nano particle (MNP) corona due to interaction is an often-overlooked aspect drug design nano-medicine. Mostly, MNP-Protein studies focus on the toxicity particles (NPs) biological environment analyze biocompatibility. However, preserving functional specificity proteins NP becomes critical for effective translation This paper investigates non-classical between insulin ZnO MNPs using classical electrical characterization technique at GHz frequency with objective understand effect (MP) nanoparticle (NP) morphology characteristics therefore protein. The was subjected thermal enzymatic (papain) perturbation study denaturation Experimental results demonstrate that plays important role insulin.